Secretion of Prolactin Following Three Dimensional Culture of Human Endometrial Tissue

Introduction: Endometriosis is the presence of endometrial glandular and stromal cells outside of the uterine cavity. This disease is found in about 10% of women of the reproductive age and in up to 50% of women with infertility. Surgery continues as the first-line treatment to eradicate endometriotic lesions but recurrence of the condition occurs in up to 47% of women. Materials and Methods: The aim of the present study is to determine prolactin levels in human culture of endometrial fragment. Endometrial biopsy in Premenopausal patient’s women referred to Toronto Center for Advanced Reproductive Technology (T.C.A.R.T) for infertility treatment such as uterine myomas or ovarian cyst. Endometrial samples were collected from a total of ten normal ovulating women on cycle days 19-24. The biopsies were obtained from the fundal region of the uterine cavity. Ten tissue fragments were cultured by three dimensional methods for each patient. Supernatant fluid sample was collected from endometrial samples which were cultured in a three-dimensional fibrin matrix. Results: Level of prolactin in Supernatant fluid of endometrial samples were placed in a three-dimensional fibrin matrix culture system were determined. These data showed Cell proliferation was observed in 91% of the wells. Angiogenesis was observed in 51 wells that showed cell proliferation (56%). The level of Prolactin in the supernatant fluid of wells that showed angiogenesis were increased (P<0.05) compare to supernatant fluid of wells that didn’t show angiogenesis. Conclusion: Prolactin might play an important role in promoting neovascularization and cell proliferation in establishment endometriosis. Prolactin is involved in the regeneration of the endometrium and the growth of endometriosis.